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Then, we tested the independent docking of The 2 Zn2 and Zn1 fragments on the straight DNA hairpin, both simultaneously or sequentially.
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4. Resistance to significant bending is outwardly a result of the additional contacts that equally Zn fingers create, by pushing their side loops (the a.a. 17–19 for Zn1, along with the a.a one hundred twenty–122 for Zn2) deep from the DNA grooves flanking the SSB gap, which forbid further more bending from the hairpin. it may well anyway be speculated that, around a for much longer time scale compared to μs obtainable to MD simulations, these ’side contacts’ could eventually lead to a forced bending. Overall, we noticed a superb correlation from the SSB gap opening Using the DNA bending angle, together with With all the degree of regional undertwisting with the double helix.
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